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A 5-fold dilution of plasma is assayed spectrophotometrically for cholesterol - concentration of diluted plasma

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A 5-fold dilution of plasma is assayed spectrophotometrically for cholesterol.

Given the following spectrophotometic data, what is the concentration of cholesterol in the diluted plasma in micromol/mL? (DO NOT INCLUDE THE UNITS IN YOUR ANSWER)

A500 standard = 0.59
A500 sample = 0.41
C (standard) = 4.2mM

asked Oct 1, 2015 in CHEMISTRY by mclge786 Rookie

1 Answer

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Foumula for concentration of cholesterol for each sample: image.

Substitute image, image and image.

image

Concentration of each sample is 2.9186 mM.

5-fold dilution means that a solution is diluted until its molarity is one-fifth of its original value, that is, the volume of the solution is five times its original value.

image

Concentration of cholesterol in the diluted plasma is image.

answered Oct 1, 2015 by Lucy Mentor
Answer is wrong.

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